Calcium measurement in extravascular body fluids (e.g., cerebrospinal fluid, pleural fluid, peritoneal fluid, synovial fluid) quantifies free and protein-bound calcium to aid in diagnosing metabolic, inflammatory, or neoplastic processes affecting fluid compartments.
Parameter Details
Analyte Total calcium (Ca²⁺)
Specimen types Any non-blood body fluid:
• Cerebrospinal fluid (CSF)
• Pleural fluid
• Peritoneal (ascitic) fluid
• Synovial fluid
Collection & handling • Collect aseptically in a sterile, additive-free container
• Transport at 2–8 °C and analyze within 4 h
• Avoid cell lysis and contamination with blood
Assay methodology Colorimetric photometric assay (o-cresolphthalein complexone) or ion-selective electrode (ISE) measurement
Turn-around time 1–2 working days
Reference intervals Fluid-specific; typical ranges (laboratory-validated):
• CSF: 2.0–2.8 mg/dL (0.50–0.70 mmol/L)
• Pleural: 0.5–1.0 mmol/L
• Peritoneal: 0.7–1.2 mmol/L
• Synovial: similar to serum when inflammatory
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