Differential Light Scanning (DLS), often referred to as Dynamic Light Scattering, is an analytical technique used to measure the size distribution and stability of particles, colloids, and nanoparticles in suspension. It is widely applied in nanotechnology, pharmaceuticals, polymer science, food technology, and environmental studies for characterizing particle size and aggregation behavior.
DLS works by detecting fluctuations in the intensity of light scattered by particles undergoing Brownian motion in a liquid medium. These fluctuations are analyzed to calculate the hydrodynamic diameter and size distribution of the particles. It is particularly effective for sub-micron to nanometer-sized particles (1 nm to 10 µm) and provides fast, non-invasive results.
Applications include:
Nanomedicine and drug delivery: Characterizing nanoparticles, liposomes, and micelles for stability and size control.
Colloid and polymer research: Measuring particle dispersion and detecting aggregation.
Environmental science: Analyzing suspended particulates in water and wastewater.
Food and cosmetics industries: Ensuring uniformity in emulsions and suspensions.
DLS is valued for its speed, accuracy, and minimal sample preparation, making it a standard tool in research, quality control, and product development laboratories.
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