Creatine kinase (CK), also known as creatine phosphokinase (CPK), catalyzes the reversible phosphorylation of creatine, playing a key role in cellular energy homeostasis. Serum CK measurement aids in the diagnosis and monitoring of muscle injury, myocardial infarction, and certain neuromuscular disorders.
Parameter Details
Analyte Total creatine kinase activity (CK-MM, CK-MB, CK-BB isoenzymes)
Specimen type Serum (5 mL clotted blood in SST tube)
Collection & handling Separate serum within 2 h; avoid hemolysis and vigorous tourniquet use; transport at 2–8 °C; analyze within 24 h or freeze at –20 °C if delayed
Assay methodology Spectrophotometric enzymatic assay (UV-absorbance) or immunoinhibition for CK-MB isoform
Turn-around time 1 working day
Reference intervals • Total CK: 20–200 U/L (male), 20–180 U/L (female)‡
• CK-MB: < 5 ng/mL or < 5% of total CK
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