Quantitative measurement of plasma catecholamines (epinephrine and norepinephrine) aids in the evaluation of suspected pheochromocytoma, paraganglioma, and other disorders of autonomic function. Because circulating levels are normally low and fluctuate rapidly, strict pre-analytical controls and sensitive analytical methods are required.
Parameter Details
Analytes • Epinephrine (adrenaline)
• Norepinephrine (noradrenaline)
Specimen type Plasma collected with pre-chilled EDTA (or sodium metabisulfite) tube plus antioxidant stabilizers
Collection requirements • Patient resting supine for ≥ 30 minutes
• Avoid caffeine, nicotine, and certain medications for 24 h
• Draw into pre-iced tube on ice; centrifuge at 4 °C within 30 minutes
Assay methodology High-performance liquid chromatography (HPLC) with electrochemical detection or liquid chromatography–tandem mass spectrometry (LC–MS/MS)
Turn-around time 2–3 working days
Reference intervals Method- and age-specific. Typical adult supine ranges:
• Epinephrine: 0–150 pg/mL (0–0.83 nmol/L)
• Norepinephrine: 70–750 pg/mL (0.42–4.58 nmol/L)
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