Qualitative urine screening for Δ⁹-tetrahydrocannabinol (THC) metabolites detects recent cannabis exposure. The assay employs immunoassay technology to identify the presence—or absence—of THC-COOH above a predefined cutoff. Positive screens should be confirmed by a chromatographic method (e.g., GC-MS or LC-MS/MS).
Parameter Details
Analyte Δ⁹-Tetrahydrocannabinol carboxylic acid (THC-COOH)
Specimen type Spot urine (random collection)
Collection requirements 30–60 mL midstream urine in a clean, dry, specimen container; note collection time and patient identifiers
Assay methodology Enzyme-linked immunosorbent assay (ELISA) or competitive fluorescence polarization immunoassay (FPIA)
Cutoff concentration 50 ng/mL THC-COOH (SAMHSA standard; may vary by regulatory requirement)
Turn-around time 1 working day
Sample Collection Information
Volume Required: 2g
Nature of Sample: Fecal, Urine, Sputum, Powder, Liquid
Do not eat or drink anything except water for 8–12 hours before your sample collection.
Avoid alcohol and fatty foods 24 hours before your sample collection.
🔹 Urine & Stool Tests
Collect the sample in a sterile container.
Avoid taking antibiotics or medications unless advised by your doctor.
Drink plenty of water before a urine sample collection for easier collection.
🔹 Pregnancy Test (Urine or Blood hCG Test)
A morning urine sample is preferred for the most accurate results.
For blood sample, no fasting is required.
🔹 Liver & Kidney Function Tests
Avoid alcohol and high-protein foods 12–24 hours before your sample collection.
Stay hydrated and avoid excessive exercise before the your sample collection.
🔹 ECG & CardiacTests
Avoid caffeine or energy drinks at least 3 hours before your sample collection.
Wear loose, comfortable clothing for easy placement of electrodes.
🔹 X-Ray & Imaging Tests
Remove any metallic items (jewelry, belts, etc.) before your sample collection.
Inform the radiologist if you are pregnant or suspect pregnancy.
🔹Sample Transportation
Samples are to be transported in a triple package format: