Zetasizer nano-particle analyzer (series Malvern nano ZS) supplied by Malvern instruments ltd., UK was used to determine the size distribution of the given sample. The results were analyzed using standard software Dispersion technology software, version 4.00. The instrument performs size measurements using a process called Dynamic Light Scattering (DLS), also known as PCSPhoton Correlation Spectroscopy, measures brownian motion and relates this to the size of the particles. It does this by illuminating the particles with a laser and analyzing the intensity fluctuations in the scattered light. As DLS is sensitive to the intensity of light scattered by particles, and larger particles scatter more light than small particles, then the DLS is very sensitive to the presence of aggregates, and hence, this technique is an excellent basis for studying the stability of nano particle size distribution. 0.5g of sample was place into the sample holder and the place on the analysis chamber and cover it, on the cheallar and allowed it to cool to 15 degree Celsius then on the desktop computer connected to the equipment and double click on the equipment shortcuts on the desktop computer to allowed you operate the system, input all the information about the sample and Open the nitrogen follow rate 20 finally click on start to start the analysis.